The neuroprotective effect of icariin in the cell model of Parkinson's disease
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：supported by the National Natural Science Foundation of China(No30971004)
关 键 词：icariin MPP+ apoptosis MES23.5 cell
摘 要：Objective Herba Epimedii(HEP) is one of the most frequently prescribed herbs in traditional Chinese medicine formula for treatment of osteoporosis,cardiovascular diseases,and improvement of neurological functions in ***,a marker flavonoid glycoside in HEP,is believed to be the major active ingredient that account for its biological *** present study was designed to investigate the protective effect of icariin against 1-methyl- 4-phenylpyridinium(MPP)-induced neurotoxicity and the possible *** MES23.5 cells were routinely cultured and treated with icariin in the presence or absence of MPP+.MTT method was used to detect the cell *** mitochondrial membrane potential was determined by flow *** real time RT-PCR was used to detect the gene expression of Bcl-2 and *** activity of ERE promoter was detected by Dual Luciferase Assay. Results Pretreatment with icariin resulted in an enhancement of survival of MES23.5 cells against MPP-induced *** decreased the mitochondrial membrane *** pretreatment could partly reverse the toxic effect of ***,pretreatment with icariin could increase the Bcl-2 gene expression and decrease the Bax gene expression which affected by *** treatment alone could not induce the ERE promoter luciferase *** The present study clearly demonstrated that icariin has neuroprotective effects against MPP- induced neurotoxicity in MES23.5 cells,and its mechanism may be related to its anti-apoptosis eflfects.