Induced expression of polysialic acid on transplanted Schwann cells and in injured rat spinal cord promotes axon regeneration and functional recovery
作者单位：Centre for Neuroscience and TraumaQueen Mary School of Medicine and DentistryUniversity of London4 Newark StreetLondonUK Department of NeurosurgeryGuizhou Provincial People's Hospital Department of NeurosurgeryRoyal London HospitalLondonUK
会议名称：《2013年贵州省神经外科年会》
会议日期：2013年
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
摘 要：Background Providing cellular support and modifying the glial scar around the lesion are two key strategies for promoting axonal regeneration after spinal cord *** cells(SCs) are an important source of cells for neural repair;however,naive SCs have poor integration with spinal cord *** previous studies showed that over-expressing polysialic acid(PSA) on SCs by transducing them with polysialyltransferase(PST) facilitates their integration and migration in spinal *** also showed that lentiviral vector(LV) mediated PSA expression in injured spinal cord modifies the glial scar and promotes the growth of ascending sensory *** and Methods To study the PST/SCs transplantation combined with direct LV/PST injection in spinal cords after dorsal column transection to find if the combined treatments could lead to faster and profounder motor functional recovery compared with animals receiving combined GFP/SCs transplantation with control LV/GFP *** Morphological study showed significantly more injured corticospinal axons growing close to the lesion/transplant borders and into the caudal spinal cord in the PST group than in the GFP ***,there was a significant increase in serotonergic axonal sprouting around the lesion/transplants in the PST *** also found over-expressing PSA around the lesion site did not cause allodynia and hyperalgesia in our injury *** These results demonstrate the promising therapeutic benefit of over-expressing PSA in transplanted SCs and spinal cord in promoting axonal growth and restoring motor function.