Effect of Pesticides on soil microbial and enzyme activity
会议名称：《两岸三地药理学与临床药理学术会议》
会议日期：2008年
关 键 词：pesticides soil microbe soil enzyme microbial diversity
摘 要：Objective Pesticides has gain an increasing awareness because of it is becoming a serious environ- mental problem and come to threaten the health of *** effect of five pesticides (zineb,cop- force,the mixture of carbendazim and mancozeb,hymexazol) on soil bacteria,fungi,actinomyces,and Five specific enzymes were chosen for investigation (urease,dehydrogenase,invertase,acid phosphates and pro- tease).Methods The enumeration of the soil micro flora was done by the dilution plate method;The enzyme activity was determined by traditional ***-Wiener index as well as 16S rRNA-PCR amplifica- tion and DGGE fingerprinting was used for detection of shift in microbial community diversity in pesticides contaminated agricultural *** The outcome showed that the microbial diversity was significantly changed after the application of pesticides,the effect of pesticides on microbe had a order from top to bot- tom:*** Our results indicate that the use of the pesticides hymexazol resulted in an altered soil community structure,in particular for the *** was markedly in- hibited by hymexazol,zineb,carbendazim and mancozeb and the inhibiting rates were varied between 30.30 % and 21.21%;Urease activity was also inhibited significantly by hymexazol,the inhibiting rate was 37.67%;Protease activity was markedly inhibited by zineb and hymexazol,the inhibiting rates were 27.27 % and 18.18 % respectively;Phosphates activity was inhibited significantly by hymexazol,zineb,car- bendazim and mancozeb,the inhibiting rates were range from 22.12 %-3.54 %;Dehydrogenase activity was not significantly affected by ***,the correlation of all indexes were analyzed,the data sug- gested that all indexes existed certain correlation.