Functional Characterization of a Low Dose Ionizing Radiation-Induced Gene
作者单位：Laboratory of Radiation EffectKorea Institute of Radiological and Medical SciencesSeoul 139-706Korea National Radiation Emergency Medical CenterKorea Institute of Radiological and Medical SciencesSeoul 139-706Korea National Radiation Emergency Medical CenterKorea Institute of Radiological and Medical SciencesSeoul 139-706Korea Laboratory of Radiation Experimental TherapeuticsKorea Institute of Radiological and Medical SciencesSeoul 139-706Korea Laboratory of Radiation EffectKorea Institute of Radiological and Medical SciencesSeoul 139-706Korea Laboratory of Radiation EffectKorea Institute of Radiological and Medical SciencesSeoul 139-706Korea
会议名称：《The Second Asian and Oceanic Congress for Radiation Protection》
会议日期：2006年
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：ionizing radiation induced-genes double strand breakages
摘 要：Exposure of cells to ionizing radiation（IR）results in DNA double strand breakages（DSBs）.Upon DSB formation,cells induce cell cycle arrest,DNA damage repair,and cell *** investigations of cellular radiation response,complexities of the mechanisms limit our understanding of the *** that of the cellular response to low dose ionizing radiation is quite *** we tried screening and isolation of low dose ionizing radiation-induced *** were isolated based on their higher expression following 1cGy gamma irradiation compared to non-irradiated *** of the isolated genes revealed a nucleotide sequence which encodes exonuclease domain highly similar to that of Exonuclease *** vitro analysis of the gene product as a form of recombinant protein exhibited nuclease *** gene product was detected in the nucleus with other apoptotic nucleases such as CAD and AIF following *** nuclease activity and colocalization with other apoptotic nucleases support the notion that the gene product might be involved in IR-induced cell *** data,thus,suggest that the low dose IR-induced gene product acts in concert with other apoptotic nucleases to enhance apoptosis following low-dose ionizing irradiation.