Influence of Caveolin-1 on the Autophagy Induced by glucose/serum Deprivation in PC12 Cells
作者单位：Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery College of Life ScienceLiaoning Normal University Regenerative Medicine CentreFirst Affiliated Hospital of Dalian Medical University
会议名称：《中国生理学会第24届全国会员代表大会暨生理学学术大会》
会议日期：2014年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
关 键 词：Caveolin-1 Autophagy oxidative stress PC12 glucose/serum Deprivation
摘 要：Autophagy is a natural process of‘self-eating’that occurs within cells and can be either pro-survival or can cause cell *** a pro-survival mechanism,autophagy obtains energy by recycling cellular components such as macromolecules or *** response to glucose/serum Deprivation（GSD）,autophagy is induced and most of these signals converge on the kinase m TOR（mammalian target of rapamycin）.Caveolin-1 is a scaffolding/regulatory protein that interacts with diverse signaling *** studies have implicated that autophagy is associated with stromal Caveolin-1,but the mechanism is not *** the present study,we firstly established an autophagic model of PC12 cells induced by glucose/serum deprivation,the cell viability,DNA damage,[Ca2+]i（intracellular free Ca2+concentration）or MMP（mitochondrialmembrane potential）were determined using MTT,Confocal Laser Scanning Microscope and Flow Cytometry assays,*** both SH-SY5Y and PC12 cells,GSD condition significantly decreased the cell viability which was accompanied by increased intracellular reactive oxygen species production,lipid peroxidation level,and oxidative DNA *** detected therelationship between autophagy and stromal Caveolin-1,we overexpressed or knocked down Caveolin-1in nerve cells using sh RNA or M-β-CD,and detected the autophagic features of cells after GSD with Western blot,immunofluorescence and q *** results showed that Caveolin-1 promoted GSD-induced *** together,these results establish that caveolin-1 plays a central role in regulation of oxidative stress,metabolic switching,and autophagy in nerve cells,and may represent a critical target in nerve diseases.