The hyperactivity of the pro-inflammatory cytokines in the brain and spleen of chronic stressed rats
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学]
基 金：supported from the Major State Basic Research Development Program of China(No.2006CB504501) Key Technologies R&D Program of Sichuan Province(07JY029-086,2007SGY015)
关 键 词：chronic unpredictable stress pro-inflammatory cytokines RT-PCR
摘 要：正Objective Depression is a devastating neurobiological illness that afflicts an estimated 12-15%of the population at some point in life,and a variety of physiological and psychological stress may lead to *** emerging literature implicates a role for pro-inflammatory cytokines in *** this study,we observed the expression of proinflammatory cytokines including IL-1,IL-6,IL-18,TNF-αand TGF-P in the different brain regions and spleen in the chronic unpredictable stressed rats and analyzed the relationship between depression-like behaviors and the dysregulation of the pro-inflammatory *** One group of Wistar rats was exposed to a series of mild stressors such as periods of food and water deprivation,continuous lighting and stroboflash,Meanwhile,the stressors were applied continuously and randomly for 4 *** sucrose preference test and the locomotor activity were used to assess depression-like *** production of pro-inflammatory cytokines in hippocampus,cortex,hypothalamus,and spleen were determined with Reverse Transcription-Polymerase Chain Reaction（RT-PCR）.Results Chronic stress significantly decreased the sucrose preference,locomotor activity and body weight of stressed ***-6 and LL-18 mRNA levels were increased remarkably in the hippocampus;TNF-αmRNA level was elevated in the hypothalamus and cortex;IL-1 and IL-18 mRNA levels were also significantly higher in the spleen in the stressed *** The chronic unpredictable stress induced the overexpression of pro-inflammatory cytokines in some brain regions and spleen,and the hyperactivity of the pro-inflammatory factors may play a role in the pathogenesis of depression.