Rhynchophylline Vasodilation of Human Mesenteric Artery Involves L-type Calcium Channel Inhibition
会议名称：《第八届海峡两岸心血管科学研讨会》
会议日期：2011年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
摘 要：Aims Uncaria species(Gouteng in Chinese) have been used as ethnopharmacological medicines to treat ailments of the cardiovascular and central nervous *** the main alkaloid constituent of Uncaria species,rhynchophylline(Rhy) exhibits a similar pharmacological activity when compared with Gouteng and has drawn extensive attention in recent years for its antihypertensive and neuroprotective *** cardiovascular effect of Rhy has been suggested to be partly due to Ca channel ***,very little is known about the actions of Rhy on peripheral resistance vessels and whether L - type voltage - gated Ca current (I) inhibition were involved in Rhy - induced *** therefore studied the actions of Rhy on endothelium - denuded human mesenteric artery and its effect on the I in human mesenteric artery smooth muscle cells(HMASMCs).Methods Tension measurement was carried out in endothelium -denuded second - order and third - order human mesenteric arterial rings by use of chart 6.0 software via Powerlab Electrophysiolograph, the effect of Rhy on the precontracted artery was *** smooth muscle cells were enzymatically isolated from the same *** effect of Rhy were examined on Ba(10mM) currents through L - type Ca channels by use of the whole - cell perforated patch clamp ***(1) Different concentrations(1 -100μM) of Rhy relaxed(92.16±3.51%) human mesenteric artery rings precontracted with KCl(60 mM) in a dose -dependent manner,the IC50 of Rhy obtained from KCl contracted arteries was(38.26±1.12)μM(n = 8,P0.05 ).Different potassium chan