Determination of eight major bioactive components in the traditional Chinese medicinal preparation Xiaochengqi Decoction by high performance liquid chromatography

作     者：Kang Li1, Yuanyuan Wang2, and Yongguang Bi3 1) School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China 2) Colleage of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong,China 3) School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China 

会议名称：《2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(CMBB 2010)》

会议日期：2010年

学科分类：1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床] 

基　　金：supported by the National Natural Science Foundation for Young Scholars of China (Grant No. 30801515) 

关 键 词：pharmaceutical analysis Xiaochengqi decoction HPLC 

摘      要：High performance liquid chromatography was employed to determine the contents of eight marker bioactive components such as rhein, aloe-emodin, nobiletin, emodin, honokiol, magnolol, chrysophanol, and physcion in the traditional Chinese medicinal preparation, Xiaochengqi decoction. The separation was performed on a C18 column by stepwise gradient elution with water (1.1% v/v acetic acid)–acetonitrile (0.01min, 75:25;15 min, 60:40;30 min, 50:50;40 min, 45:55;55min, 30:70) as the mobile phase at a flow-rate of 1.0ml·min-1, with UV detection at 254nm. Eight regression equations showed good linear relationships between the peak area and the amount of each marker. The recoveries of the markers were 99.6, 99.5, 97.4, 97.7, 98.9, 97.9, 99.0 and 98.5%, respectively. The repeatability and reproducibility (relative standard deviation) of the method were less than 2.1% and 2.5%, respectively.