Ophiobolin B inhibits proliferation of glioblastoma U87MG cells by inducing S phase arrest and caspase-dependent apoptosis
作者单位：Department of NeurosurgeryCollege of MedicineWonkwang University College of PharmacyWonkwang University
会议名称：《中国抗癌协会神经肿瘤专业委员会第八届学术会议》
会议日期：2011年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
摘 要：*** Human glioblastoma is the most common primary malignant brain tumor that only weakly responsive to the current therapeutic ***,new therapeutic agents for treat this devastating disease are urgently *** are secondary metabolites of certain fungi belonging to the genera Bipolaris,Drechslera,Cephalosporium and *** sesterterpene-type compounds（C25） are characterized by a unique tricyclic chemical *** B（OPB） is a minor member of a class of phytotoxic metabolites and acts as an inhibitor of calmodulin action in calcium *** has a wide range of biochemical and pharmacological effects,including antitumor activities,but its mechanism of action is not clearly *** this study,we investigated that the relationship between the antiproliferative activities of berberine and the apoptotic pathway associated with its molecular mechanism of action in human glioblastoma U87MG *** and mathods The effect of berberine on cell viability was quantified using MTT *** cytometric analysis was used to measure the cellular DNA *** blot analysis was performed using primary antibodies against p21,p27,cyclin-dependent kinase（CDK）2,CDK4,cyclin B,cyclin A,cyclin E,Bcl-2,Bax,caspase-9,caspase-3,poly（ADPribose） polymerase（PARP）,and *** nuclei of U87MG cells were stained with DAPI in order to assess the degree of *** data shown are a summary of the results from at least three experiments and statistical evaluation of the results was performed by One-way analysis of variance（ANOVA）.*** OPB significantly decreased the cell viability of U87MG cells in a dose-dependent *** cytometry showed a dose dependent increase of S phase arrested after treatment with OPB in U87MG *** blot analysis showed that the OPB-induced S phase arrest was mediated through the increased expression of cyclin-dependent kinase inhibitor（CDKi） proteins（p21 and p27） a