MiR-124 regulates early neurogenesis in the optic vesicle and forebrain,targeting NeuroDl
作者单位：The State Key Laboratory of Brain and Cognitive ScienceInstitute of BiophysicsChinese Academy of Sciences15Datun RoadChaoyang District Department of NeurologyLurie Comprehensive Cancer CenterCenter for Genetic MedicineNorthwestern University Feinberg School of Medicine303 E.SuperiorChicagoIL 60611USA
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：MiR-124 NeuroD1 optic vesicle cell proliferation neurogenesis Xenopus laevis
摘 要：正MicroRNAs（miRNAs） are involved in the fine control of cell proliferation and differentiation during the development of the nervous ***-124,a neural specific miRNA,is expressed from the beginning of eye development in Xenopus and has been shown to repress cell proliferation in the optic ***,its role at earlier developmental stages is *** we show that miR-124 exerts a differential role in cell proliferation at the optic vesicle stage,the stage which precedes the optic cup *** show that miR-124 is both necessary and sufficient to promote cell proliferation and repress neurogenesis at the optic vesicle stage,playing an anti-neural role. Loss of miR-124 upregulates expression of neural markers NCAM,N-tubulin while gain of miR-124 downregulates these ***,miR-124 interacts with a conserved miR-124 binding site in the 3’UTR of NeuroD1 and negatively regulates expression of the proneural marker NeuroD1,a bHLH transcription factor for neuronal *** miR-124-induced effect on cell proliferation can be antagonized by *** results reveal a novel regulatory role of miR-124 in neural development and uncover a previously unknown interaction between NeuroD1 and miR-124.