环境水样中8种氟喹诺酮类抗生素亲和萃取与毛细管电泳质谱分析
作者单位：北京分子科学国家实验室北京大学化学学院 中国农科院武汉油料研究所
会议名称：《第二十届全国色谱学术报告会及仪器展览会》
会议日期：2015年
学科分类：082803[工学-农业生物环境与能源工程] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0828[工学-农业工程] 070302[理学-分析化学] 0804[工学-仪器科学与技术] 0703[理学-化学]
关 键 词：fluoroquinolone CE-MS immunoaffinity extraction broad-specific antibody environmental water
摘 要：Fluoroquinolones（FQs） are a widely used class of synthetic antibiotics with broad activity spectrum of mycoplasma and bacteria（both Gram positive and negative）. Due to the extensive use of FQs in veterinary science and pharmaceutical industry, they are expected to enter the environmental water via animal excretion and hospital waste, giving rise to a constant need for a sensitive and reliable method to monitor FQs in aquatic environment. Thus, an off-line procedure of immunoaffinity extraction（IAE） followed by capillary electrophoresis-mass spectroscopy（CE-MS） has been developed for simultaneous determination of eight typical FQs in environment water. A broad-specific polyclonal antibody was home-prepared and immobilized on a Sepharose 4B stationary phase for sample cleanup, FQs extraction and pre-concentration. CE-MS was subsequently applied for FQs separation and quantification. The immunoaffinity column was evaluated in the parameters of selectivity, binding capacity, elution protocol, and recovery. The separation and detection by CE-MS were also investigated. Under the optimized conditions, the developed method was finally applied to analyze FQs in environmental water sample from a local lake, providing satisfactory recoveries from 72% to 112% and LODs ranging from 1.2 n M to 5.0 n M.