Genotyping of Enterocytozoon bieneusi in sheep and cattle in northeast China and zoonotic importance
作者单位：College of Veterinary MedicineNortheast Agricultural University
会议名称：《中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会》
会议日期：2015年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
基 金：中国博士后科学基金第7批特别资助项目(2014T70307) 黑龙江省博士后第5批特别资助项目(LBH-TZ0502)
关 键 词：Enterocytozoon bieneusi Sheep Cattle Genotyping Zoonosis Host-Range Expansion
摘 要：This study investigated fecal specimens from 489 sheep and 537 cattle in multiple cities in northeast China for the prevalence and genetic characteristics of Enterocytozoon bieneusi by PCR and sequencing of the ribosomal internal transcribed ***-eight sheep specimens(13.9%) and 32 cattle specimens(6.0%) were positive for *** polymorphisms enabled the identification of 9known genotypes(BEB4,BEB6,CM7,CS-4,EbpC,G,I,J,and OEB1) and 11 new genotypes(NESH1to NESH6 and NECA1 to NECA5).The genotypes formed two genetic clusters in a phylogenetic analysis,with CS-4,EbpC,G,NESH1 to NESH3,and NECA1 to NECA5 distributed in zoonotic group 1 and BEB4,BEB6,CM7,I,J,OEB1,and NESH4 to NESH6 distributed in potentially host-adapted group *** 70%of cases of *** infections in sheep were contributed by human-pathogenic genotypes BEB6,CS-4,and EbpC,and over 80%of those in cattle were by genotypes BEB4,CS-4,EbpC,Ⅰ,and *** co-occurrence of genotypes BEB4,CS-4,EbpC,I,and J in domestic ruminants and children in northeast China and the identification of BEB6 and EbpC in humans and water in central China imply the possibility of zoonotic *** study also summarizes *** genotypes obtained from ruminants worldwide and displays their host ranges,geographical distributions,and phylogenetic *** data suggest a host range expansion in some group 2 genotypes(notably BEB4,BEB6,I,and J) that were previously considered to be adapted to *** should be concerned about the increasing zoonotic importance of group 2 genotypes with low host specificity.