Sialylation of epidermal growth factor receptor regulates chemosensitivity to gefitinib in ovarian cancer cells
作者单位：The First Affiliated Hospital of Wenzhou Medical University
会议名称：《2013年浙江省妇科肿瘤学术年会暨省级医学继续教育项目“宫颈癌筛查及子宫颈疾病规范化诊治”学习班》
会议日期：2013年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
摘 要：Objectives To evaluate the Sialylation of epidermal growth factor receptor regulates chemosensitivity to gefitinib in ovarian cancer *** In order to understand the effects of ST6Gal-I on cell behavior,OVCAR3 cells were stably-transduced with ST6Gal-I,and then were detected using RT-PCR,Immunofluorescence and Western *** MTT method was used to examine the effects of ST6Gal-I on chemosensitivity in OVCAR3 ***/p-FAK, Paxillin/P-Paxillin,ERK/P-ERK,AKT/P-AKT responses were compared in parental and ST6Gal-I-expressing ***(1)This study conformed geftinib have significant cytotoxic activity in a dose-and-time-dependent manner(p0.05).Suppression of P-FAK,pAKT and p-ERK activation in cells treated with gefitinib was observed and suggests the role of gefitinib inhibition of proliferative cell signaling pathway(P0.05).(2)As expected,ST6Gall mRNA and protein levels were higher in OVCAR3-ST6Gall cells then OVCAR3,suppression of both phosphorylated and non-phosphorylated of FAK、AKT and ERK were ***,the anticancer effect of the EGFR kinase inhibitor,gefitinib,was decreased in OVCAR3-ST6Gall cells(P0.05). Conclusion Geftinib has significant cytotoxic *** of ST6Gal I decreased the cytotoxic effect of gefitinib to OVCAR3 cells, and FAK-PI3K/AKT signaling pathway may be involved.