cDNA Microarray Analysis of the 1080 Genes Expression Profiling during different Segments in Moniezia expansa
会议名称：《中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会》
会议日期：2009年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
关 键 词：cDNA Microarray Genes Expression Profiling Moniezia expansa
摘 要：The gene expression profilings in different proglottids of *** were analysed with cDNA microarray of 1080 ESTs from the worm.A total of 4,056 spots including full length and partial complementary DNAs representing novel,known,and control genes were *** showed that 55 genes expression in immature proglottids,134 genes in mature proglottids and 103 genes in gravid proglottids were up-regulated,and that 7 genes in immature proglottids,68 genes in mature proglottids and 78 genes in gravid proglottids were down-regulated compared with control(scolex-neck proglottids).Major function of these genes are about metabolism,transport,protein biosynthesis,transcription factors,apoptosis,cell differentiation,cell communication and nucleic acid binding protein -related *** changes of some genes,such as UBE2A,Carβ,RAD51,DAZ,PKAc and 2 unknown genes were confirmed by Real-time quantitative Polymerase Chain Reaction(RT-PCR).Results provide a frame of gene expression changes in different development proglottids of ***,which may be helpful in understanding the pathogenesis of the worm and studying its genes concerned with reproductive organs development as a model organism.