Primary culture of basolateral amygdala neurons of newborn rat
会议名称:《中国神经科学学会第四次会员代表大会暨第七届全国学术会议》
会议日期:2007年
学科分类:0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071006[理学-神经生物学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
关 键 词:cell culture the basolateral amygdala neurons
摘 要:正The basolateral amygdala is a brain region with critical roles in emotional control and fear *** understand the basolateral amygdala physiology and pathology,it is important to study its synaptic transmission and ***,technical difficulties exist in dissecting basolateral amygdala *** overcome this problem,we have developed a novel dissection procedure in which we can gain the basolateral amygdala ***,we describe a reliable method to prepare primary cultures of P0 rat basolateral amygdala *** of cultures can take as little as 2 *** can be maintained for as long as 2 *** neurons survived better and showed healthy ***, the present study suggest that primary culture of the basolateral amygdala neurons is a powerful tool to study neuronal synaptic transmission and modulation.