Bee venom-induced changes in expression of NMD A receptor subunits in spinal cord,SI area and hippocampus of the rat:an immunohistochemical study
会议名称：《中国神经科学学会第四次会员代表大会暨第七届全国学术会议》
会议日期：2007年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：immunohistochemistry NMDA receptor subunit spinal cord S1 area hippocampus bee venom
摘 要：正Using tissue immunohistochemistry technique,the present study investigated the effects of subcutaneous（s.c.） bee venom（BV） administration on the expression of three major NMDA receptor subunits（NR1,NR2A,NR2B） in rat lumbar spinal cord,S1 area and *** to *** injection（100 ug/50 ul） into the hind paw,the animal exhibited a typical behavioral expression of persistent pain and hyperalgesia,whereas *** of equal volume of normal saline produced little *** coronal sections of lumbar spinal cord and brain were sequentially prepared at 4 h after BV or saline *** was then performed and positive labeling was visualized by treatment with diaminobenzidine（DAB） and hydrogen *** of NMDA receptor subunits expression were finished by comparisons between two sides of the hindpaw or comparisons between B V-treated and saline-treated groups. As shown in our results,expression of the NR2A subunit of NMDA receptor was significantly exaggerated in ipsilateral lumbar spinal cord,S1 area and hippocampus following *** but not saline *** significant changes were detected in the expression of NR1 and NR2B subunits in each ***,the number of NR2A-positive dendric tree also markedly increased in ipsilateral hippocampus of BV-treated ***,these data suggest that the NR2A subunit of NMDA receptor might behave as a candidate molecular detector in mediating nociceptive signal processing in the lumbar spinal cord,S1 area and hippocampus following BV-induced persistent pain and *** is also speculated that BV-evoked augmentation of NR2A-positive dendric tree in the hippocampus may bear some associative relationships with the synaptic plasticity occurred in this area.