ROS does not contribute to acute development of nitroglycerine tolerance in rat aortas
作者单位：Institute of Vascular Medicine and School of Biomedical Sciences Chinese University of Hong Kong
会议名称：《中国病理生理学会第九届全国代表大会及学术会议》
会议日期：2010年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by CUHK Focused Investment scheme
摘 要：正The development of nitroglycerine tolerance limits its clinical efficacy of nitric oxide（NO）*** cellular mechanisms have been proposed to explain this phenomenon,one of which is an exaggerated production of reactive oxygen specious（ROS）.The present study examined whether ROS contribute to the occurrence of nitroglycerine tolerance which is induced acutely in aortas isolated from male Sprague-Dawley *** aortas were exposed to 30μmol/L nitroglycerine（with and without pre-incubation of ROS inhibitors） for 90 min and washed out 4 times during the following 60 min before phenylephrine was added to induce a steady contraction. Subsequently,nitroglycerine was added cumulatively（3 nmol/L-50μmol/L）to evoke ***-induced relaxations were severely impaired after 90-min pre-exposure to *** with apocynin,tempol and tiron plus DETCA