Proteomic analysis reveal toxin biosynthesis related proteins in toxin-producing Alexandrium tamarense
会议名称：《中国藻类学会第八次会员大会暨第十六次学术讨论会》
会议日期：2011年
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
关 键 词：A.tamarense CI01 Cell cycle Toxin Biosynthesis Proteome
摘 要：正This research optimized the synchronization method of Alexandrium tamarense, defined the cell cycle phases with flow cytometry and detected toxin content and composition variation during the whole cell ***,compared the protein expressions from *** at different toxin biosynthesis phases（T24,T26,T34） used 2-DE.A total of 120 differentially expressed protein spots（2 fold） were found and identified using MALDI-TOF/TOF,nine of which showed a roughly 3 fold in toxin decreased *** as S adenosylhomocysteine hydrolase,alcohol dehydrogenase, sulfotransferase,and arginine deiminas might be involved in toxin biosynthesis and could be used as potential biomarkers in PSP producing ***,the toxin biosynthesis pathways in *** were *** results are a first step in the dissection of the behavior of the dinoflagellate *** proteome during cell cycle and may provide new insights into the relationship between dinoflagellate blooms and toxin biosynthesis within the cell cycle.