DNA DAMAGES INDUCED BY MIXED PARTICLE FIELD IRRADIATION

作     者：Guo Huijun~1, Liu Luxiang~(1*), Zhao Linshu~1, Li Jiacai~2, Zhao Shirong~1, Wang Jing~1(1. Key Lab of Agricultural Nuclear Technology & Aerospace Breeding, Ministry of Agriculture/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081 2. Institute of High Energy Physics, CAS, Beijing 100093) 

会议名称：《全国作物生物技术与诱变技术学术研讨会》

会议日期：2005年

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This research was supported by NSFC(30270345) National 863 Project (2002AA241011), the IAEA projects RC12610 and RAS5040 

摘      要：正 Cosmic rays is one of the most important components in aerospace, which could be used to induce mutation in plants. However, little is known about its damage effect to DNA sequence. In this study, mixed high-energy particles generated by accelerator were used to simulate secondary cosmic rays （CR）, and the damage effects of plasmid DNA were preliminarily analyzed. PUC18 plasmid DNA was prepared and irradiated by mixed high-energy particles generated by accelerator to simulate secondary cosmic rays （CR）, including pion meson, muon, positron, electron, photon and proton, with the dosages of 68Gy, 128Gy, 190Gy, 248Gy and 518Gy. Irradiated DNA and the non-irradiated control