Genetic variation of TAP gene and the outcomes of hepatitis C virus infection in drug users

作     者：SHI Chao,XIA Xian,ZHANG Yong-xiang,YU Rong-bin,SU Jing, CUI Qian,WANG Lu,WANG Ning 1 Department of Epidemiology and Biostatistics, School of Public Health,Nanjing Medical University, Nanjing 210029,China 2 Department of Infection Control and Prevention, General Hospital of Beijing Military Command, Beijing 100700,China 3 Department of Infectious Diseases,The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,China 4 Department of Epidemiology,National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100050,China 

会议名称：《华东地区第十次流行病学学术会议暨华东地区流行病学学术会议20周年庆典》

会议日期：2010年

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 100205[医学-精神病与精神卫生学] 10[医学] 

基　　金：supported by the National Mega-project of Science Research(2008ZX10002 -013 and 2009ZX1004-904) 

关 键 词：Hepatitis C virus Infection Outcome Drug users(DUs) TAP gene Polymorphism 

摘      要：Objective Transporter associated with antigen processing(TAP) has essential roles in the antigenpresenting systems,translocating antigenic peptides from the cytosol into the endoplasmic reticulum. Genetic polymorphisms of TAP gene may be involved in the outcomes of HCV infection.A casecontrol study was conducted to test this *** Total 173 drug users(DUs) with HCV persistent infection,189 DUs with HCV self-limiting infection,and 225 DUs with HCV uninfection were obtained during the one-year period(2006.01-2006.12).Antibodies to HCV antigens were detected by enzyme-linked *** presence of viral particles in the serum was determined by reverse -transcriptase polymerase chain reaction(RT-PCR).The genotypes of six polymorphisms of TAP gene were analyzed by ARMS-PCR *** Of the six polymorphisms,one of which(TAP2-379) was observed to have statistically significant association with recovered HCV infection(P0.05),two of which(TAP1-637 and TAP2-379) were observed to have statistically significant association with persistent HCV infection(P0.05),and one of which(TAP1-637) was observed to have statistically significant association with outcomes of HCV infection(P0.05).The extended haplotype constructed with TAP polymorphisms was analyzed among the healthy control,recovered and persistent *** TAP2 haplotype H(Ile-Ala-Arg-Thr) and K(Ile-Thr-Arg-Thr) were shown to present significant difference between the recovered and control groups(P0.05).TAP1 haplotype B(Ile-Gly),D(Val -Gly),and TAP2 haplotype E(Val-Thr-Arg-Thr),H(De-Ala-Arg-Thr) were shown to present significant differences between the persistent and control groups(P0.05).The frequency of TAP1 haplotype B(De-Gly) or D(Val-Gly) was found increased significantly in persistent group,in comparison with that in recovered group(P0.05).Conclusions These findings suggest a potential role of TAP2 gene as a candidate gene for susceptibility to HCV *** the genetic polymorphism