BACTERICIDAL EFFECT OF PEROXYNITRITE ON HELICOBACTER PYLORI:A MORPHOLOGICAL STUDY
作者单位：Baskent University Faculty of Medicine.Departments of PharmacologyAnkara Turkey Baskent University Faculty of Medicine Departments of Microbiology Ankara Turkey Baskent University Faculty of Medicine Departments of 3Histology Ankara Turkey Baskent University Faculty of Medicine Departments of Pharmacology Ankara Turkey Baskent University Faculty of Medicine Departments of Pharmacology Ankara Turkey Baskent University Faculty of Medicine Departments of MicrobiologyAnkara Turkey
会议名称：《第十五届国际药理学大会》
会议日期：2006年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by B. U. Research Grant (DA 05/31)
关 键 词：peroxynitrite Helicobacter pylori
摘 要：正 INOS activity is elevated in the antrum and fundus of duodenal ulcer patients infected with Helicobacter pylori. We aimed to investigate the time - and concentration - dependent bactericidal and morphological effects of peroxynitrite （PN;ONOO- ） on H. *** PN was synthesized as quenched - flow method. A stock culture of H. pylori NCTC 11637 was exposed to different concentrations of PN （4×10-2-10-4） or decomposed PN or fresh medium. Samples were taken at 0. 15, 30, 60, and 120 minutes, for the evaluation of viable bacteria, bacterial morphology with gram strain and transmission electron *** PN exposure the number of viable bacteria decreased within the first 15 minutes . The morphological conversion of replicating spiral form to viable but nonreplicatingcoccoid form, and bacterial lysis were found to be concentration dependent. Decomposed PN showed no bactericidal activity against H. pylori.