Preparation and Characterization of Monoclonal Antibodies against Cryptosporidium Andersoni

作     者：Fuchun Jian Longxian Zhang Jingliang Su Changshen Ning Nan Liang College of Animal Husbandry and Veterinary Henan Agricultural university Zhengzhou 45002 College of Animal Medicine China Agricultural university Beijing 100094 China 

会议名称：《12th International Congress of Protozoology》

会议日期：2005年

学科分类：090602[农学-预防兽医学] 09[农学] 0906[农学-兽医学] 

摘      要：正The oocyts of a Cryptosporidium strain, ***, which was isolated on the feces by sucrose sugar solution and purified by discontinuous sucrose gradients, were treated by filtration and centrifugation, and were then freeze-thawed and sonicated. Balb/c mice were immunized for four times with these purified oocyst antigens of C. andersoni. Spleen cells of the mice were selected to fuse with SP 2 / 0 cells. The positive wells were selected by indirect ELISA, and after 3 to 4 generations of cloning by limited dilution, 5