Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells
作者单位：Institute of AndrologyNanjing University of Chinese Medicine State Key Laboratory of Reproductive MedicineClinical Center of Reproductive Medicineand the First Affiliated HospitalNanjing Medical University
会议名称：《中华中医药学会第十四次男科学术大会》
会议日期：2014年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the Chinese National Natural Science Foundation(NO.81273760) a Postgraduatelnnovation Program of the Jiangsu Province Education Department (NO.CXLX13_583)
关 键 词：YC extract GC-1 spg cells self-renewal Gfral PI3K
摘 要：Objective:To investigate the effect of Yangjing Capsule(养精胶囊,YC)extract on proliferation of GC-1 spg cells and the ***:GC-1 spg cells were treated with different concentrations of YC *** assay was performed to detect the cell *** cytometry was used to measure the cell cycle and apoptosis of GC-1 spg ***-time PCR and Western blot were applied to determine the mRNA and protein expression of Oct-4 and ***α1knockdown and LY294002(PI3K inhibitor)were applied to explore the underlying ***:After 48 h treatment of YC,the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced *** showed YC could evidently stimulate the proliferation of GC-1 spg *** increased mRNA and protein expression of Oct-4 and PLZF suggested YC extract could promote self-renewal of GC-1 spg *** Gfrα1 siRNAs and LY294002 treatments could hold back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf,and consequently,inhibit the proliferation of GC-1 spg cells induced by YC ***:YC extract could stimulate the proliferation of GC-1 spg *** binding to Gfrα1,YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.