Effects of dexmedetomidine on excitatory synaptic transmission in rat spinal dorsal horn
作者单位：Institute of NeurobiologyInstitutes of Brain Science and State Key Laboratory of Medical NeurobiologyFudan University Cell Electrophysiology LaboratoryWannan Medical College
会议名称：《中国神经科学学会第十届全国学术会议》
会议日期：2013年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：AMPA receptor NMDA receptor LTP dexmedetomidine spinal cord
摘 要：Objective To investigate cellular mechanisms underlying the analgesic effects of dexmedetomidine,a selective α2-adrenergic receptor agonist,at spinal *** The whole-cell patch clamp recording and field potential recording were performed on the superficial dorsal horn（SDH,laminae HI） of spinal cord slices with attached dorsal root from juvenile（2-3 weeks） *** synaptic transmission（eEPSCs） and long-term potentiation（LTP）of C-fiber evoked field potentials（fEPSPs） were recorded from SDH neurons by stimulating dorsal root（DR） and Lissauer’s tract（LT） *** We found that eEPSC were rapidly and dose-dependently reduced by bath application of *** amplitude and frequency of spontaneous EPSC（sEPSC）,exogenous N-methyl-D-aspartate（NMDA）-induced currents and α-amino-3-hydroxy-5-methyl-4-isoxazolepropri-anate（AMPA）-induced currents were all ***,slices pretreated with dexmedetomidine showed failed LTP induction compared with control *** These data suggest that dexmedetomidine possibly inhibits excitatory synaptic transmission in rat spinal dorsal horn through pre-synaptic and post-synaptic mechanisms.