Celastrol induces apoptosis in human hepatocellular carcinoma Bel-7402 cells through both mitochondria- and Fas/FasL-mediated pathways
作者单位：Institute of Clinical PharmacologyAnhui Medical UniversityKey Laboratory of Anti-inflammatory and Immunopharmacology of Education Ministry ofChina
会议名称：《安徽省药理学会2012年学术年会》
会议日期：2013年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100214[医学-肿瘤学] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Celastrol,Apoptosis,Mitochondria,Fas/FasL,P53 HCC
摘 要：Aims To elucidate whether celastrol,an active ingredient of the traditional Chinese medicinal plant Tripterygium Wilfordii,induces apoptosis in Bel-7402 cells and further to explore the underling molecular *** Bel-7402 cells were treated with celastrol at different *** viability of Bel-7402 cells was measured using the MTT assay. Fluorescence microscope was used to observe the morphological changes of Bel-7402 cells. AnnexinV/ PI staining and TUNEL assay were utilized to detect apoptosis and DNA fragmentations of Bel-7402 cells *** protein levels were measured by western *** Celastrol caused a dose-dependent growth inhibition and induced apoptosis of Bel-7402 ***-induced apoptosis was characterized by cleavage of caspase-3,caspase-8 and caspase-9 protein,increased Fas and FasL ***,celastrol induced the release of cytochrome *** also up-regulated the expression of Bax,down-regulated the expression of *** addition,Celastrol up-regulated the expression of P53 through inhibited the expression of *** These data indicated that celastrol could induce apoptosis of Bel-7402 cells,which may be partly mediated through activation of both mitochondria- and Fas-mediated pathways.