Molecular cloning,biochemical and biophysical studies of dihydrodipicolinate reductase of Pseudomonas aeruginosa PAO1
作者单位：Department of BiotechnologyPanjab universityChandigarhIndia Bioinformatics CentreInstitute of Microbial TechnologyChandigarhIndia Department of BiochemistryPanjab UniversityChandigarhIndia Department of BiophysicsAll India Institute of Medical SciencesNew DelhiIndia Centre for Microbial BiotechnologyPanjab UniversityChandigarhIndia
会议名称：《第十七届国际生物物理大会;第十二届中国生物物理大会》
会议日期：2011年
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学]
关 键 词：dihydrodipicolinate reductase
摘 要：Dihydrodipicolinate reductase(DHDPR,E.C.1.***.1.26) is one of the key enzyme of the lysine biosynthetic pathway present in bacteria forming L-lysine and diaminopimelate(DAP).It is considered to be a potential drug target because bacteria immediately get lysed in the absence of DAP while it is absent in *** we have cloned,overexpressed and studied biochemical and biophysical properties of dihydrodipicolinate reductase (dapB) of Pseudomonas aeruginosa(Pa) *** was purified to homogeneity using nickel affinity