CRL4Wdr70 facilitates chromatin recruitment of Exo1 to promote long-range resection of double strand breaks

作     者：曾鸣 Laifeng Ren Ken'Ichi Mizuno Konstantinos Nestoras Haibin Wang Zizhi Tang Xiaobo Wang Liandi Guo Daochun Kong Qun He Lilin Du Antony M.Carr Cong Liu 

作者单位：Sussex Centre for Genome Damage and Stability CentreSchool of Life SciencesUniversity of Sussex West China Second University HospitalSichuan University 

会议名称：《2014年第七届中国模式真菌研讨会》

会议日期：2014年

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

摘      要：Homologous recombination(HR)is important for repairing potentially lethal DNA double strand breaks(DSBs)in the context of *** are resected by the combination action of the Mrell-Rad50-Nbsl(MRN),Ctpl,Exol and Rqhl-Top3-Dna2 factors to form the ssDNA substrates which initiate *** these DNA processing enzymes navigate nucleosome-packaged DNA remains poorly *** find that the fission yeast DCAF protein,Wdr70,is recruited to DSBs in complex with Ddbl and the Cullin4 ubiquitin ligase(CRL4).CRL4recruitment stimulates H2B lysine 119 mono-ubiquitination(uH2B)of chromatin both proximal