Life at the single molecule level:Imaging and sequencing individual molecules in single cells
作者单位：Department of Chemistry and Chemical BiologyHarvard University
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 071007[理学-遗传学]
摘 要：正Recent advances in single-molecule imaging in living cells allow quantitative and system-wide descriptions of gene expression and regulation with single molecule sensitivity.It was found that low probability events of single molecules can have important biological consequences,such as the change of a cellular phenotype.This has everything to do with the fact that DNA are single molecules in individual cells.Meanwhile,recent advances in high throughput DNA sequencing have allowed sequencing the genome and transcriptome of a single human cell.The combination of single molecule and single cell imaging and sequencing offers exciting possibilities for biology.