Expression,purification of human DOPA decarboxylase and the establishment of high-throughput inhibitors screening model
作者单位：Key Laboratory of Exploration and Utilization of Aquatic Genetic ResourcesMinistry of EducationCollege of Fisheries and Life ScienceShanghai Ocean University Shanghai Center for System BiomedicineShanghai Jiao tong University
会议名称：《全国第九届海洋生物技术与创新药物学术会议》
会议日期：2014年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：Supported by the National Natural Science Funds for the Young Scholar Foundation of China under Grant NO.81102377 Shanghai Universities First-class Disciplines Project(Discipline Name:Marine Science) the National Key Technology Research and Develop Program(2012BAC07B03)
关 键 词：Dopa decarboxylase Parkinson’s disease Inhibitors High-throughput screening model
摘 要：Objective:Parkinson’s disease is one of common nervous system degenerative diseases.Dopa decarboxylase(DDC)has been thought as one of the drug target for Parkinson’s disease,but there is lacking of a high-throughput screening inhibitors model for dopa decarboxylase.Therefore,our study aims to establish a high-throughput screening inhibitors model.Method:Cloning,expression and purification of DDC and PEPC which is to test the activity of DDC in the coupling reaction.CO content is detected,based on a series of enzyme-linked reactions.Result:The pure enzymes of DDC and PEPC were obtained.We set up a high-throughput screening model for inhibitors of DDC,and 2 inhibitors were found from 70 natural compound product.Conclusion:A suitable high-throughput screening model is set up and two inhibitors of dopa decarboxylase have been found.