玉米YL蛋白质与ZmPEPCs互作及对其酶活性的影响
作者单位：华中农业大学
学位级别：硕士
导师姓名：张方东
授予年度：2023年
主 题：Maize yl gene Yeast two-hybrid BiFC PEPC Prokaryotic expression Enzymatic properties
摘 要：Maize（Zea mays L.）is one of the important crops,which plays an important role in national economy and biological *** are important materials for studying plant growth and *** this study,candidate gene GRMZM2G095254（yl gene）of yellow leaf mutant（yellow leaf,yl）and Weaky mutant（Weaky）was *** on the interaction between YL protein and CPEPC,CPEPC was previously verified in the *** study explored the interaction between Root-PEPC and YL protein,and studied the influence of the interaction between YL protein and Zm PEPCs on their enzymatic *** main results are as follows:*** the amino acid sequences of CPEPC,CPEPC,Root-PEPC and Bacterial-PEPC in maize,it was found that the amino acid sequences of the three plant type PEPCs have high homology,but the amino acid sequences of plant type PEPCs and bacterial type PEPCs have low homology,and there are significant differences in gene *** structural differences of the four PEPCs amino acid sequences may be related to the differences in the catalytic and regulatory properties of their *** two-hybrid and BiFC experiments have shown that there is interaction between Root-PEPC and YL protein,with interaction sites located in the nucleus and weak interaction signals on the cell *** protein,CPEPC,CPEPC and Root-PEPC were expressed in Escherichia *** enzyme activity of Zm PEPCs was measured at different temperatures with YL *** results showed that the enzyme activities of CPEPC,CPEPC and Root-PEPC were all highest at 30℃.After adding YL protein and ATP simultaneously to the PEPC enzyme activity measurement system,the enzyme activity of Zm PEPCs was significantly decreased under various temperature *** protein can inhibited the enzyme activity of Zm PEPCs,and the inhibitory effect of YL protein was an active process,and its activation required ATP *** the PEPC enzyme activity of the leaves in the two-le
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