Apoptosis of T-lymphocytes and the Activation of NF-kB in Severe Acute Pancreatitis
作者单位：天津医科大学
学位级别：硕士
导师姓名：YOU SHENG YI
授予年度：2004年
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
主 题：Severe acute pancreatitis apoptosis NF-kB Lymphocytes
摘 要：Background:There is clear evidence that, because of the development of humantechnology, and changes of the food intake (Hyperlipidemia,Hypercalcemia, alcohol abuse);Severe Acute Pancreatitis (SAP) isbecoming common in the countries world *** is also becoming a serious disease in intensive care unit (ICU)and in the department of surgery causing high morbidity and ***:The purpose of this study was to investigate theimmunopathological changes, the involvement of the multipleorgan dysfunction syndromes (MODS) and the relationship withthe activation of the NF-kB in *** and results:86 rats were used, 22 were sham operated group and 64 were dividedinto SAP models group and treatment *** 86 animals study allowed us to evaluate the immunopathologicalchanges;the involvement of distant organs and the effect of NF-kBactivation in severe acute pancreatitis (SAP). In the sham operated animals, there was no death, both the pancreas and the lungs Structure were normal. The percentage of CD4+ T-lymphocytes (39.49±2.98) and CD8+ T-lymphocytes (20.49±2.68) were high. Whereas the activation of NF-kB was *** animals from SAP group were investigated 24 hours after SAP induction by duodenopancreaticobiliary injection of sodium deoxycholate, the results were as below:There were 4 deaths in SAP models group. Histologically, the boundary of pancreas was not clear, and neutrophil infiltrations in the alveoli were present. The percentage of CD4+ T-lymphocyte (27.79±2.80%) was lower than that of sham operated group (39.42±2.86%) statistically p0.01. The ratio of CD4+/CD8+ T-lymphocytes in SAP group (0.88±0.11%) was also significantly lower than that of the sham operated group (1.94±0.13%) with P value 0.05. It indicates cells mediated immunity defective in *** assessed NF-kB activation in rat of SAP induced by injection of sodium deoxycholate into CBD, 3 hours after the episode of SAP, immunohistochemistry examination show: the per
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