Inhibitory effect of oxymatrine on serum hepatitis B virus DNA in HBV transgenic mice
Inhibitory effect of oxymatrine on serum hepatitis B virus DNA in HBV transgenic mice作者机构：ShanghaiInstituteofDigestiveDiseaseRenjiHospitalShanghaiSecondMedicalUniversityShanghai200001China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2004年第10卷第8期
页 面：1176-1179页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 100602[医学-中西医结合临床] 10[医学]
基 金：Supported by the Key Project of Shanghai Medical Development Foundation,(No:99ZDI001) grants from 1999 Youth Liver Diseases Foundation of Chinese Liver Diseases Association
主 题：氧化苦参碱 血清学检查 药理作用 乙型病毒性肝炎 转基因技术 动物模型
摘 要：AIM:To study the inhibitory effect of oxymatrine on serum hepatitis B virus (HBV) DNA in HBV transgenic mice. METHODS:HBV transgenic mice model was established by microinjection,and identified by HBV DNA integration and *** mice with replicating HBV were divided into 3 groups,and injected with normal saline (group A,n=9),50 mglkg (group B,n=8) and 100 mg/kg (group C,n=9) oxymatrine intraperitoneally once a day for 30d,*** of serum HBV DNA in HBV transgenic mice was performed by competitive polymerase chain reaction (PCR) in combination with DNA hybridization quantitative detection technique before and after treatment. RESULTS:Compared with pre-treatment,the serum HBV DNA in group A (F=1.04,P=0.9612) and group B (F=1.13, P=0.8739) had no changes after ***,in group C serum HBV DNA was significantly decreased (F=13.97, P=0.0012).The serum HBV DNA after treatment was lower in group C than in groups B and A (F=8.65,P=0.0068; F=12.35,P=0.0018;respectively).The serum HBV DNA after treatment was lower in group B than in group A,but there was no statistical significance (F=1.43,P=0.652). CONCLUSION:Oxymatrine has inhibitory effects on serum HBV DNA in HBV transgenic mice.
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