Hyposmotic membrane stretch potentiated muscarinic receptor agonist-induced depolarization of membrane potential in guinea-pig gastric myocytes
Hyposmotic membrane stretch potentiated muscarinic receptor agonist-induced depolarization of membrane potential in guinea-pig gastric myocytes作者机构：Department of Physioloy Yanbian University College of Medicine Juzi 121 Yanji 133000 Jilin Province China. wenxiexu@***
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2002年第8卷第4期
页 面：724-727页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：the National Natural Science Foundation of China No.39860031
主 题：胃肌细胞 毒蕈碱型胆碱受体 去极现象 低渗性膈膜牵张 动物实验
摘 要：AIM: To investigate the relationship betweenhyposmotic membrane stretch and muscarinic receptoragonist-induced depolarization of membrane potentialin antral gastric circular myocytes of ***: Using whole cell patch-clamp techniquerecorded membrane potential and current in singlegastric myocytes isolated by collagena ***: Hyposmotic membrane stretch hyperpolarizedmembrane potential from -60.0mV±1.0mV to -67.9mV±1.0mV. TEA (10mmol/L), a nonselective potassiumchannel blocker significantly inhibited hyposmoticmembrane stretch-induced hyperpolarization. After KCIin the pipette and NaCI in the external solution werereplaced by CsCI to block the potassium current,hyposmotic membrane stretch depolarized the membranepotential from -60.0 mV±-1.0mV to -44.8 mV±2.3mV(P0.05), and atropine (1 pmol/L) inhibited thedepolarization of the membrane potential. Muscarinicreceptor agonist Carbachol depolarized membranepotential from -60.0mV±1.0mV to -50.3 mV±0.3mV(P0.05) and hyposmotic membrane stretchpotentiated the depolarization. Carbachol inducedmuscarinic current (Icch) was greatly increased byhyposmotic membrane ***: Hyposmotic membrane stretchpotentiated muscarinic receptor agonist-induceddepolarization of membrane potential, which is relatedto hyposmotic membrane stretch-induced increase ofmuscarinic current.
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