Identification of noncoding RNA-encoded proteins on lipid droplets

作     者：Ting Huang Adekunle T.Bamigbade Shimeng Xu Yaqin Deng Kang Xie Ololade O.Ogunsade Ahmed Hammad Mirza Jifeng Wang Pingsheng Liu Shuyan Zhang 黄婷;Adekunle T.Bamigbade;徐式孟;邓亚琴;解康;Ololade O.Ogunsade;Ahmed Hammad Mirza;王继峰;刘平生;张淑妍

作者机构：School of Basic Medical SciencesSouthwest Medical UniversityLuzhou 646000China National Laboratory of BiomacromoleculesCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2021年第66卷第4期

页      面：314-318,M0003页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the National Key Research Development Program of China(2018YFA0800900,2016YFA0500100,and 2018YFA0800700) the National Natural Science Foundation of China(31671402,91954108,91857201,31671233,31701018,and U1702288) the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12040218) 

主　　题：代谢疾病 非编码RNA 中性脂 质谱学 骨骼肌细胞 脂质代谢 磷脂膜 细胞脂质 

摘      要：With the rapid development of high-throughput sequencing technologies,it has recently become evident that the eukaryotic genome is extensively transcribed[1,2].Large-scale projects for the systematic annotation and functional characterization of genes,such as GENCODE and FANTOM,have demonstrated that at least 80%of mammalian genomic DNA is actively *** has become increasingly clear that many genomic regions classified as noncoding actually harbor short open reading frames(sORFs)with fewer than 300 nucleotides,encoding small,functional proteins or *** small proteins have evaded detection due to their small size,low abundance,instability,and loss during sample preparation[3].However,emerging experimental techniques,including ribosome profiling and mass spectrometry,along with computational advances have enabled their discovery and analysis[3,4).Most have been identified in prokaryotic organisms[4],but a growing number have also been discovered in eukaryotic organisms,including plants,zebrafish,insects,mice,and humans[2-4].These proteins have been found to play important roles in many fundamental biological processes[3]and have been reported to localize to cellular compartments such as mitochondria[5],late endosomes/lysosomes[6],and endoplasmic reticulum[7].However,given the large number of putative proteins encoded by noncoding RNAs,there is a need for much more *** particular,there is a need for more information on the subcellular localization of these proteins,which may provide insight into their biological functions.