Expression of neurofilament gene in spinal motoneurons during neural regeneration
Expression of neurofilament gene in spinal motoneurons during neural regeneration作者机构:Acad Mil Med Sci Inst Basic Med Sci Beijing 100850 Peoples R China Beijing Med Coll PLA Beijing 100071 Peoples R China
出 版 物:《Chinese Science Bulletin》 (中国科学通报)
年 卷 期:1999年第44卷第3期
页 面:225-229页
核心收录:
学科分类:1001[医学-基础医学(可授医学、理学学位)] 10[医学]
主 题:neurofilament mRNA neural regeneration spinal motoneurons in situ hybridization rat.
摘 要:The technique in situ hybridization was used to measure the levels of light (NF-L),medium(NF-M) and heavy (NF-H) neurofilament protein subunits mRNA in L4-6 spinal motoneurons in adult rat during regeneration following a unilateral crush of the sciatic *** was found that the hybridization signals of each neurofilament subunit mRNA were dramatically decreased in spinal motoneurons postaxotomy by light microscopy. The hybridization signals of NF-L and NF-M mRNA were located in cytoplasm of neurons, whereas NF-H mRNA was found in both nucleus and cytoplasm of neurons. lmage analysis showed that the encoding levels of mRNA for each of neurofilament subunit mRNA reduced on the 3rd d and returned to control levels on the 28th d following the lesion. The relative levels of mRNA coding for each neurofilament subunit were significantly different. The lowest level of NF-L mRNA was observed at 5 d postaxotomy, and that of NF-M, NF-H mRNA on the 7th and 10th d after injury. Moreover,the levels of HF-M and