Characterization of the bacterial communities associated with biofilters in two full-scale recirculating aquaculture systems

作     者：Yuexin MA Xin DU Yubin LIU Tao ZHANG Yue WANG Saisai ZHANG Yuexin MA;Xin DU;Yubin LIU;Tao ZHANG;Yue WANG;Saisai ZHANG

作者机构：Key Laboratory of Environment Controlled AquacultureMinistry of EducationDalian 116023China Dalian Modern Agricultural Production Development Service CenterDalian 116023China Dalian Tianzheng Industry Company LimitedDalian 116011China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2021年第39卷第3期

页      面：1143-1150页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 0707[理学-海洋科学] 09[农学] 0815[工学-水利工程] 

基　　金：Supported by the National Key R&D Program of China(No.2017YFD0701700) National Natural Science Foundation of China(Nos.31472312,31672673) 

主　　题：biofilters bacterial community recirculating aquaculture system Illumina-MiSeq sequencing 

摘      要：Bacteria play a major role in metabolizing ammonia and other metabolites in recirculating aquaculture systems(RASs).To characterize and compare the bacterial communities in the biofilters of two full-scale RASs for the culture of puffer fish,Takifugu rubripes,at different ages and densities were *** overall,47807 optimized reads of the 16 S rRNA gene with V4-V5 region were obtained from four biofilm samples collected after biofilm *** 97%cut-off level,these sequences were clustered into 500 operational taxonomic units,and were classified into 19 bacterial phyla and 138 *** the phylum level,Proteobacteria and Bacteroidetes were the most abundant,followed by Nitrospirae and *** the genus level,Colwellia,Marinifilum,Oceanospirillum,Lutibacter,Winogradskyella,Pseudoalteromonas,Arcobacter,and Phaeobacter were the top *** and Nitrospira were main ammonia-and nitrite-oxidizing *** in bacterial communities at different sampling dates and similarities of both biofilters were revealed in the Venn diagram and cluster *** a good water quality and health offarmed fish in RASs depended on the correct management of the bacterial *** study provides more accurate information on the bacterial communities associated with the bifilters of both RASs.