Salting-out Assisted Liquid-Liquid Extraction for Analysis of Caffeine and Nicotinic Acid in Coffee by HPLC-UV/Vis Detector
作者机构：Department of ChemistryCollege of Natural SciencesJimma UniversityP.O.Box 378JimmaEthiopia Forest and Climate Change AuthorityOromia EnvironmentP.O.Box 10633Finifinne/Addis AbabaEthiopia
出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)
年 卷 期：2020年第4卷第4期
页 面：298-306页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
基 金：Jimma University Chemistry Department (Jimma Ethiopia) is greatly acknowledged for providing laboratory facilities and chemicals for this work
主 题：Raw and roasted coffee Caffeine Nicotinic acid SALLE HPLC-UV/Vis
摘 要：A salting-out assisted liquid-liquid extraction method followed by high performance liquid chromatography with ultravio-let-visible detector(SALLE-HPLC-UV/Vis)has been proposed for determination of caffeine and nicotinic acid in raw and roasted coffee *** parameters affecting chromatographic separations including mobile phase composition,injec-tion volume,flow rate and column temperature were *** parameters affecting the extraction efficiency of SALLE method such as type and volume of the organic solvent,type and amount of salt,pH of the sample,and concentration of the ion-pairing reagent were also studied and thereby optimum conditions were *** calibration curves which were constructed at five different concentration levels using the optimum conditions exhibited good linearity,with the coef-ficient of determination(R2)0.999 and 0.995 for caffeine and nicotinic acid,*** limits of detection(LOD)and quantification(LOQ)which were determined at 3 and 10 times signal-to-noise ratio were 0.05 and 0.13 mg/L for nicotinic acid and 0.20 and 0.63 mg/L for caffeine,***-and inter-day precision studies were demonstrated satisfactory precision with RSD values below *** recoveries were also studied,and their results were ranging from 82-122%for both raw and roasted coffee *** findings demonstrated that the proposed method could be used as an effective and best alternative for the determination of caffeine and nicotinic acid in raw and roasted coffee samples.
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