Identification of key gene networks controlling organic acid and sugar metabolism during watermelon fruit development by integrating metabolic phenotypes and gene expression profiles

作     者：Muhammad Jawad Umer Luqman Bin Safdar Haileslassie Gebremeskel Shengjie Zhao Pingli Yuan Hongju Zhu M.O.Kaseb Muhammad Anees Xuqiang Lu Nan He Chengsheng Gong Wenge Liu Muhammad Jawad Umer;Luqman Bin Safdar;Haileslassie Gebremeske;Shengjie Zhao;Pingli Yuan;Hongju Zhu;M.O.Kaseb;Muhammad Anees;Xuqiang Lu;Nan He;Chengsheng Gong;Wenge Liu

作者机构：Zhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesHenan Joint International Research Laboratory of South Asian Fruits and CucurbitsZhengzhouChina Key Laboratory of Biology and Genetics Improvement of Oil CropsOil Crops Research InstituteChinese Academy of Agricultural SciencesMinistry of Agriculture and Rural AffairsWuhan 430062China 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2020年第7卷第1期

页      面：191-203页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2016-ZFRI)National Key R&D Program of China(2018YFD0100704) the China Agriculture Research System(CARS-25-03) National Natural Science Foundation of China[31672178&31471893] Scientific and Technological Project of Henan Province(202102110197) 

主　　题：metabolism sugar networks 

摘      要：The organoleptic qualities of watermelon fruit are defined by the sugar and organic acid contents,which undergo considerable variations during development and *** molecular mechanisms underlying these variations remain *** this study,we used transcriptome profiles to investigate the coexpression patterns of gene networks associated with sugar and organic acid *** identified 3 gene networks/modules containing 2443 genes highly correlated with sugars and organic *** these modules,based on intramodular significance and Reverse Transcription Quantitative polymerase chain reaction(RT-qPCR),we identified 7 genes involved in the metabolism of sugars and organic *** these genes,Cla97C01G000640,Cla97C05G087120 and Cla97C01G018840(r^(2)=0.83 with glucose content)were identified as sugar transporters(SWEET,EDR6 and STP)and Cla97C03G064990(r^(2)=0.92 with sucrose content)was identified as a sucrose synthase from information available for other ***,Cla97C07G128420,Cla97C03G068240 and Cla97C01G008870,having strong correlations with malic(r^(2)=0.75)and citric acid(r^(2)=0.85),were annotated as malate and citrate transporters(ALMT7,CS,and ICDH).The expression profiles of these 7 genes in diverse watermelon genotypes revealed consistent patterns of expression variation in various types of *** findings add significantly to our existing knowledge of sugar and organic acid metabolism in watermelon.