Analysis of Survivin Expression in the Subtypes of Lymphoma

作     者：顾霞 林汉良 邵建勇 张萌 梁惠珍 Gu xia;LIN Hanliang;SHAO Jianyong;ZHANG Men;LIANG Huizhen

作者机构：广州中山大学基础医学院病理教研室510089 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2005年第4卷第4期

页      面：238-243页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：survivin lymphoma classification reverse transcription-polymerase chain reaction 

摘      要：Objective： To detect the expression of survivin in subtypes of lymphoma and its value in classifying subtypes of lymphoma. Methods： Paraffin-embedded samples collected from 219 cases of lymphoma and 13 cases of lymph node reactive proliferation from affiliated hospitals of Sun Yat-sen University during 2001-2003 were examined for the expression of survivin by using immunohistochemical staining. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of survivin in K562, HL60, Raji, and Jurkat cell lines. Semi-quantitative assay was used to evaluate the quantity of survivin protein and mRNA expression in subtypes of lymphoma. Results： Protein expression of survivin was high and strong in diffuse large B-cell lymphoma （DLBL） （88.6%, 70/79）, Burkitt lymphoma （BL） （100%, 2/2）, and lymphoblastic lymphoma （LBL） （92.3%, 12/13）, while their expression was always lower and weaker in follicular lymphoma （FL） （18.2%）, extranodal marginal zone B-cell lymphoma of mucosaassociated lymphoid tissue （MALT lymphoma） （40.9%） and marginal zone lymphoma （MZL） （33.3%）. There was a significant difference between the higher expression group （DLBL, BL and LBL） and lower one （FL, MZL, and MALT） in the expression of survivin （Chi-square test, X^2=24.77, P〈0.01）. Almost all of Reed-Sternberg cells （R-S cells） in Hodgkin lymphoma （HL） strongly expressed survivin. The protein expression of survivin was positively correlated with mRNA （r=0.6270, P〈0.01）. Conclusion： The expression level of survivin mRNA and protein shows significant difference in subtypes of lymphoma. The expression of survivin mRNA might act as a biomarker to classify the subtypes of lymphoma.