Effect of saikosaponin A on Treg and Th17 immune balance in depressive rats

作     者：Xu-Tong Guo Ji-Dong An Jian-Qiang Mei GUO Xu-tong;AN Ji-dong;MEI Jian-qiang

作者机构：Emergency department of Hebei Hospital of traditional Chinese Medicine 

出 版 物：《Journal of Hainan Medical University》 (海南医学院学报（英文版）)

年 卷 期：2020年第26卷第22期

页      面：6-9页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Hebei Science and technology program 2017 Hebei key R&D plan big health service and biomedical special project(No.17277782D) 

主　　题：Saikosaponin A Depression Th17/Treg cell Cytokines 

摘      要：Objective:To investigate the Effect of saikosaponin A on Treg and Th17 immune balance in depressive ***:The rat depression model was established with reference to the Katz method,and the rats were randomly divided into control group,model group,western medicine group,and saikosaponin A *** western medicine group was given 1.2 mg/kg/d of fluoxetine,and the saikosaponin A group was given 25 mg/kg/d of saikosaponin A,while the control group and model group were given the same volume of normal *** evaluation of depression in Rats was analyzed by Openfield-test and sugar water preference *** cytometry was used to detect the expression of Th17 and Treg *** the expression of IL-17,IL-23,TNF-α,IL-10,TGF-βwere detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the control group,the horizontal exercise score,vertical exercise score,and sugar preference of the model group decreased significantly(P0.05).Compared with the model group,the above indicators were significantly increased in the western medicine group and saikosaponin A group(P0.05).Flow cytometry showed that compared with the control group,the Th17 cells,Th17/Treg cell ratio in model group increased significantly,whereas the Treg cells decreased significantly(P0.05).Compared with the model group,The Th17 cells and Th17/Treg ratio in western medicine group and saikosaponin A group decreased,while the Treg cells increased significantly(P0.05).ELISA showed that compared with control group,the serum levels of IL-17,IL-23 and TNF-αin model group increased,while the levels of IL-10 and TGF-βdecreased(P0.05).Compared with model group,the levels of IL-17,IL-23 and TNF-αdecreased,while the levels of IL-10 and TGF-βincreased in western medicine group and saikosaponin A group(P0.05).Conclusion:Saikosaponin A can reduce the degree of depression by regulating the imbalance of Th17/Treg cells and the secretion of inflammatory cytokines in depressed rats.