Morphological and molecular characterization of Acanthamoeba isolatedfrom contact lens paraphernalia in Malaysia: Highlighting the pathogenicpotential of T4 genotype

作     者：Rosnani Hanim Mohd Hussain Nur Syahirah Mohamad Isa Khairul Ameera Kamaruddin Mohamed Kamel Abdul Ghani Naveed Ahmed Khan Ruqaiyyah Siddiqui Tengku ShahrulAnuar Rosnani Hanim Mohd Hussain;Nur Syahirah Mohamad Isa;Khairul Ameera Kamaruddin;Mohamed Kamel Abdul Ghani;Naveed Ahmed Khan;Ruqaiyyah Siddiqui;Tengku Shahrul Anuar

作者机构：Centre of Medical Laboratory TechnologyFaculty of Health SciencesUniversiti Teknologi MARAPuncak Alam CampusSelangorMalaysia Programme of Biomedical ScienceSchool of Diagnostic and Applied Health SciencesUniversiti Kebangsaan MalaysiaJalan Raja Muda Abdul AzizKuala LumpurMalaysia Department of BiologyChemistry and Environmental SciencesCollege of Arts and SciencesAmerican University of SharjahUniversity CitySharjahUnited Arab Emirates Integrative Pharmacogenomics InstituteUniversiti Teknologi MARAPuncak Alam CampusSelangorMalaysia 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2020年第13卷第12期

页      面：542-549页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Integrative Pharmacogenomics Institute(241910/2017/TSA/13) Geran Inisiatif Penyeliaan 600-IRMI 5/3/GIP(024/2019) Universiti Teknologi MARA,Malaysia 

主　　题：Morphological prevention potential 

摘      要：Objective:To determine the morphological and molecular characterization of Acanthamoeba isolates from contact lens paraphernalia in Malaysia and to investigate their pathogenic potential based on the physiological ***:One hundred and eighty contact lens wearers donated their contact lens,lens storage cases and lens solutions between2018 and *** samples were inoculated onto 1.5%nonnutrient agar plates for 14 *** chain reaction(PCR)was performed and the amplified PCR products were sequenced and compared with the published sequences in *** pathogenic potential of positive isolates was further tested using temperature-tolerance and osmo-tolerance *** species were categorized into three distinct morphological groups established by Pussard and ***:Acanthamoeba was successfully isolated from 14(7.8%)culture-positive samples in which 11 belong to morphological groupⅡand 3 belong to morphological groupⅢ,*** sequencing of 18 S ribosomal RNA gene led to the identification of the T4 genotype in all the isolated *** vitro assays revealed that 9(64.3%)Acanthamoeba isolates were able to grow at 42℃and 1 M mannitol and were thus considered to be highly ***:To the best of our knowledge,this is the first report identifying the Acanthamoeba genotype and their pathogenic potential among contact lens wearers in *** potentially pathogenic T4 genotype isolated in this study is the most predominant genotype responsible for human ocular infection ***,increasing attention should be aimed at the prevention of contamination by Acanthamoeba and the disinfection of contact lens paraphernalia.