Pharmacogenetic study of Asn680Ser and-29A>G in FSHR gene in Chinese women undergoing controlled ovarian hyperstimulation

作     者：Xiaohe Sun Tianxiang Ni Guangyu Li Jingjing Jiang Junhao Yan Zi-Jiang Chen Xiaohe Sun;Tianxiang Ni;Guangyu Li;Jingjing Jiang;Junhao Yan;Zi-Jiang Chen

作者机构：Center for Reproductive MedicineShandong UniversityThe Key Laboratory of Reproductive Endocrinology(Shandong University)Ministry of EducationShandong Provincial Key Laboratory of Reproductive Medicine National Research Center for Assisted Reproductive Technology and Reproductive GeneticsJinanShandong Province Center for Reproductive MedicineRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai Key Laboratory for Assisted Reproduction and Reproductive GeneticsShanghai Assisted Reproduction UnitDepartment of Obstetrics and GynecologySir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityKey Laboratory of Reproductive Dysfunction Management of Zhejiang ProvinceHangzhouZhejiang ProvinceChina 

出 版 物：《Journal of Bio-X Research》 (生物组学研究杂志（英文）)

年 卷 期：2018年第1卷第3期

页      面：147-152页

学科分类：10[医学] 

基　　金：supported by the National Key Research and Development Program of China(No.2016YFC1000202) by the National Natural Science Foundation of China(No.81671522). 

主　　题：controlled ovarian hyperstimulation FSH FSHR ovarian response SNP 

摘      要：The outcome of controlled ovarian hyperstimulation(COH)is various and unpredictable.According to previous studies,2 single nucleotide polymorphisms,Asn680Ser and-29A/G,have a pharmacogenetic association with ovarian response to COH.However,studies on the Asn680Ser polymorphism have yielded inconsistent conclusions and only a few studies with small sample sizes have been performed on-29A/G.The association of these 2 polymorphisms with ovarian response remains unclear.The present study evaluated the association of Asn680Ser and-29A/G genotypes with COH.A total of 414 Chinese women undergoing in vitro fertilization-embryo transfer were included.Genotypes for these single nucleotide polymorphisms were identified by high-resolution melting-curve analysis.The value of exogenous follicle-stimulating hormone dosage per oocyte divided by the body surface area(Dosage/Oocyte
Surface)was calculated for each patient as an indicator of ovarian response.The results of statistical analyses showed no association between Asn680Ser genotype and ovarian response.As for-29A/G,heterozygote individuals had more oocytes retrieved(P=0.034).Combinatorial analysis of these 2 single nucleotide polymorphisms showed that genotype A/G-Asn/Asn had lower basal-follicle-stimulating hormone and more oocytes retrieved.Analysis of genotype association with ovarian response also revealed this genotype had a significantly higher risk of developing hyper response(OR=7.86;95%CI:1.31-9.43).To some extent,there were associations between the studied polymorphisms and ovarian response;however,the power of this link is weak and has limited value for clinical prediction.