3D microfluidic gradient generator for combination antimicrobial susceptibility testing

作     者：Eric Sweet Brenda Yang Joshua Chen Reed Vickerman Yujui Lin Alison Long Eric Jacobs Tinglin Wu Camille Mercier Ryan Jew Yash Attal Siyang Liu Andrew Chang Liwei Lin Eric Sweet;Brenda Yang;Joshua Chen;Reed Vickerman;Yujui Lin;Alison Long;Eric Jacobs;Tinglin Wu;Camille Mercier;Ryan Jew;Yash Attal;Siyang Liu;Andrew Chang;Liwei Lin

作者机构：Department of Mechanical EngineeringUniversity of CaliforniaBerkeleyCA 94720USA Berkeley Sensor and Actuator CenterBerkeleyCA 94720USA Department of BioengineeringUniversity of CaliforniaBerkeleyCA 94720USA Department of Materials Science and EngineeringUniversity of CaliforniaBerkeleyCA 94720USA 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2020年第6卷第1期

页      面：229-242页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This project was funded through the Berkeley Sensors and Actuators Center(BSAC)at UC Berkeley 

主　　题：gradient testing fluid 

摘      要：Microfluidic concentration gradient generators(µ-CGGs)have been utilized to identify optimal drug compositions through antimicrobial susceptibility testing(AST)for the treatment of antimicrobial-resistant(AMR)***µ-CGGs fabricated via photolithography-based micromachining processes,however,are fundamentally limited to two-dimensional fluidic routing,such that only two distinct antimicrobial drugs can be tested at *** work addresses this limitation by employing Multijet-3D-printed microchannel networks capable of fluidic routing in three dimensions to generate symmetric multidrug concentration *** three-fluid gradient generation characteristics of the fabricated 3Dµ-CGG prototype were quantified through both theoretical simulations and experimental ***,the antimicrobial effects of three highly clinically relevant antibiotic drugs,tetracycline,ciprofloxacin,and amikacin,were evaluated via experimental single-antibiotic minimum inhibitory concentration(MIC)and pairwise and three-way antibiotic combination drug screening(CDS)studies against model antibiotic-resistant Escherichia coli *** such,this 3Dµ-CGG platform has great potential to enable expedited combination AST screening for various biomedical and diagnostic applications.