An improved draft genome sequence of hybrid Populus alba×Populus glandulosa

作     者：Xiong Huang Song Chen Xiaopeng Peng Eun-Kyung Bae Xinren Dai Guiming Liu Guanzheng Qu Jae-Heung Ko Hyoshin Lee Su Chen Quanzi Li Mengzhu Lu Xiong Huang;Song Chen;Xiaopeng Peng;Eun-Kyung Bae;Xinren Dai;Guiming Liu;Guanzheng Qu;Jae-Heung Ko;Hyoshin Lee;Su Chen;Quanzi Li;Mengzhu Lu

作者机构：State Key Laboratory of Tree Genetics and BreedingChinese Academy of ForestryBeijing 100091People’s Republic of China Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbin 150040People’s Republic of China Division of Forest BiotechnologyNational Institute of Forest ScienceSuwon 441847Republic of Korea Agro-Biotechnology Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097People’s Republic of China Department of Plant&Environmental New ResourcesKyung Hee UniversityYongin 446701Republic of Korea 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2021年第32卷第4期

页      面：1663-1672页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：supported by grants CAFYBB2017ZY001 and TGB2016001 from Fundamental Research Funds of the Chinese Academy of Forestry 

主　　题：Genome assembly Gene annotation Hybrid poplar Populus alba×p.glandulosa cl.84 K 

摘      要：Populus alba × *** clone 84 K,derived from South Korea,is widely cultivated in China and used as a model in the molecular research of woody plants because of hi gh gene transformation ***,we combined63-fold coverage Illumina short reads and 126-fold coverage PacBio long reads to assemble the *** to the hi gh heterozygosity level at 2.1% estimated by k-mer analysis,we exploited TrioCanu for genome *** PacBio clean subreads of *** × *** were separated into two parts according to the similarities,compared with the parental genomes of *** and *** two parts of the subreads were assembled to two sets of subgenomes comprising subgenome A(405.31 Mb,from ***)and subgenome G(376.05 Mb,from ***) with the contig N50 size of 5.43 Mb and 2.15 Mb,respectively.A high-quality *** × *** genome assembly was *** genome size was 781.36 Mb with the contig N50 size of 3.66 Mb and the longest contig was 19.47 *** addition,a total of 176.95 Mb(43.7%),152.37 Mb(40.5%)of repetitive elements were identified and a total of 38,701 and 38,449 protein-coding genes were predicted in subgenomes A and G,*** functional annotation,96.98% of subgenome A and 96.96% of subgenome G genes were annotated with public *** de novo assembled genome will facilitate systematic and comprehensive study,such as multi-omics analysis,in the model tree *** X ***.