Decellularized peripheral nerve grafts by a modified protocol for repair of rat sciatic nerve injury

作     者：Arash Zaminy Sara Sayad-Fathi Farshad Moharrami Kasmaie Zohreh Jahromi Adib Zendedel Arash Zaminy;Sara Sayad-Fathi;Farshad Moharrami Kasmaie;Zohreh Jahromi;Adib Zendedel

作者机构：Neuroscience Research CenterSchool of MedicineGuilan University of Medical SciencesRashtIran Student Research CommitteeSchool of MedicineGuilan University of Medical SciencesRashtIran Institute of NeuroanatomyFaculty of MedicineRheinisch-Westfälische Technische Hochschule(RWTH)Aachen UniversityAachenGermany 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第6期

页      面：1086-1092页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the Research and Technology Chancellor of Guilan University of Medical Sciences(No.95110202 to AZa). 

主　　题：acellular scaffold axonotmesis bovine crush injury detergent rat sciatic nerve xenograft 

摘      要：Studies have shown that acellular nerve xenografts do not require immunosuppression and use of acellular nerve xenografts for repair of peripheral nerve injury is safe and effective.However,there is currently no widely accepted standard chemical decellularization method.The purpose of this study is to investigate the efficiency of bovine-derived nerves decellularized by the modified Hudson’s protocol in the repair of rat sciatic nerve injury.In the modified Hudson’s protocol,Triton X-200 was replaced by Triton X-100,and DNase and RNase were used to prepare accelular nerve xenografts.The efficiency of bovine-derived nerves decellularized by the modified Hudson’s protocol was tested in vitro by hematoxylin&eosin,Alcian blue,Masson’s trichrome,and Luxol fast blue staining,immunohistochemistry,and biochemical assays.The decellularization approach excluded cells,myelin,and axons of nerve xenografts,without affecting the organization of nerve xenografts.The decellularized nerve xenograft was used to bridge a 7 mm-long sciatic nerve defect to evaluate its efficiency in the repair of peripheral nerve injury.At 8 weeks after transplantation,sciatic function index in rats subjected to transplantation of acellular nerve xenograft was similar to that in rats undergoing transplantation of nerve allograft.Morphological analysis revealed that there were a large amount of regenerated myelinated axons in acellular nerve xenograft;the number of Schwann cells in the acellular nerve xenograft was similar to that in the nerve allograft.These findings suggest that acellular nerve xenografts prepared by the modified Hudson’s protocol can be used for repair of peripheral nerve injury.This study was approved by the Research Ethics Committee,Research and Technology Chancellor of Guilan University of Medical Sciences,Iran(approval No.IR.GUMS.REC.1395.332)on February 11,2017.