Meibomian gland dysfunction model induced with complete Freund’s adjuvant in C57BL/6 mice

作     者：Yang Lu Yue Yin Lan Gong 

作者机构：Department of Ophthalmology and Vision ScienceEye&ENT Hospital of Fudan UniversityShanghai 200031China 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2020年第13卷第11期

页      面：1705-1712页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China(No.81700797) Science and Technology Commission of Shanghai Municipality(No.17411961800) 

主　　题：meibomian gland dysfunction complete Freund's adjuvant inflammation acinar atrophy intense pulsed light 

摘      要：AIM:To establish a new inflammatory animal model of meibomian gland dysfunction(MGD)in C57BL/6 ***:C57BL/6 mice were randomly divided into complete Freund’s adjuvant(CFA)group(14 animals,14 eyes),naphthazolin hydrochloride(NH)group(14 animals,14 eyes)and control group(14 animals,14 eyes).In CFA group,CFA was used in eyelid conjunctiva injection;in NH group,NH eye drops were used twice a day;control group was injected with equal dose of saline at the same time point and same site with animals in CFA *** meibomian gland orifices score(MGOS)was evaluated on a scale of 0 to 3 in the middle five meibomian gland orifices of the upper and lower eyelid using slit *** the successful induction of each animal model,intense pulsed light(IPL)was introduced on each mouse in CFA and NH *** red O(ORO),hematoxylin and eosin(H&E)staining were performed before and after successful induction of CFA,NH and control ***:At 12wk after CFA injection,inflammatory cell infiltration and fiber necrosis was observed,with acinar density and duct dilatation significantly lower compared with control *** NH group,the meibomian gland acini were relatively smaller and deformed compared with control group,the number of meibomian gland acini was also slightly *** inflammatory cell or fiber necrosis was observed in NH *** three times of IPL treatment(5/10 mice in each group,and the other 5 mice served as non-IPL control),MGOS was significantly lower in IPL-treated mice in NH group(P0.01).After three times of IPL treatment,the MGOS of NH group was significantly lower than that in the CFA group(P0.01).CONCLUSION:We develop a novel animal model that studies the role of inflammation in the development of MGD and IPL *** model indicates that persistent inflammatory state may be the cause of MGD and weaken the therapeutic effect of IPL.