Sesamol Alleviates the Cytotoxic Effect of Cyclophosphamide on Normal Human Lung WI-38 Cells via Suppressing RAGE/NF-κB/Autophagy Signaling

作     者：Soad Z.El-Emam Soad Z.EI-Emam

作者机构：Department of Pharmacology and ToxicologyFaculty of PharmacyOctober 6 University6 October CityGiza 12566Egypt 

出 版 物：《Natural Products and Bioprospecting》 (应用天然产物（英文）)

年 卷 期：2021年第11卷第3期

页      面：333-343页

学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 0832[工学-食品科学与工程（可授工学、农学学位）] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 0901[农学-作物学] 0902[农学-园艺学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This research did not receive any specific grant from funding agencies in the public commercial or not-for-profit sectors 

主　　题：Cyclophosphamide Sesamol Lung toxicity Autophagy Oxidative stress RAGE 

摘      要：Cyclophosphamide(CYL)is a chemotherapeutic medication commonly used in managing various malignancies like breast cancer or ***,CYL has been documented to induce lung *** of CYL toxicity is through oxidative stress and the release of damage-associated molecular patterns(DAMPs).Sesamol(SES)is a natural antioxidant isolated from Sesamum indicum and its effect against CYL-induced lung toxicity is not studied *** study aims to inves-tigate whether SES could prevent any deleterious effects induced by CYL on lung using normal human lung cells,WI-38 cell line,without suppressing its *** were pretreated with SES and/or CYL for 24 h,then cell viability was estimated by MTS and trypan blue *** mode of cell death was determined by AO/EB ***,caspase-3 level,oxidative stress,and inflammatory markers were evaluated by colorimetric and ELISA ***-PCR was performed to evaluate RAGE,NF-κB,and Beclin-1 ***-treated WI-38 cells developed a significantly increased cell death with enhanced oxidative and RAGE/NF-κb/Autophagy signaling,which were all attenuated after pretreatment with ***,we concluded that SES offered a protective role against CYL-induced lung injury via suppressing oxidative stress and RAGE/NF-κB/Autophagy signaling,which is a natural safe therapeutic option against CYL toxicities.