Function identification of Md TIR1 in apple root growth benefited from the predicted Md PPI network

作     者：Lin Liu Zipeng Yu Yang Xu Cheng Guo Lei Zhang Changai Wu Guodong Yang Jinguang Huang Kang Yan Huairui Shu Chengchao Zheng Shizhong Zhang Lin Liu;Zipeng Yu;Yang Xu;Cheng Guo;Lei Zhang;Changai Wu;Guodong Yang;Jinguang Huang;Kang Yan;Huairui Shu;Chengchao Zheng;Shizhong Zhang

作者机构：State Key Laboratory of Crop BiologyShandong Agricultural UniversityTaiʼan 271018China Shandong Peanut Research InstituteShandong Academy of Agricultural SciencesQingdao 266100China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第4期

页      面：723-736页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China(31972357,31901574,and 31772254) the National Key R&D Program of China(2019YFD1000104) 

主　　题：Apple auxin hormone Md TIR1 PPI network root growth 

摘      要：Protein–protein interaction(PPI)network analysis is an effective method to identify key proteins during plant development,especially in species for which basic molecular research is lacking,such as apple(Malus domestica).Here,an MdPPI network containing 30806 PPIs was inferred in apple and its quality and reliability were rigorously ***,a rootgrowth subnetwork was extracted to screen for critical proteins in root *** hormone-related proteins occupied the largest proportion of critical proteins,a hormonerelated sub-subnetwork was further extracted from the root-growth *** these proteins,auxin-related *** TRANSPORT INHIBITOR RESISTANT 1(MdTIR1)served as the central,high-degree node,implying that this protein exerts essential roles in root ***,transgenic apple roots overexpressing an MdTIR1 transgene displayed increased primary root *** analysis showed that MdTIR1 significantly upregulated auxin-responsive genes in apple roots,indicating that it mediates root growth in an auxin-dependent *** experimental validation revealed that MdTIR1 interacted with and accelerated the degradation of MdIAA28,MdIAA43,***,MdTIR1-mediated degradation of MdIAAs is critical in auxin signal transduction and root growth regulation in ***,our network analysis and high-degree node screening provide a novel research technique for more generally characterizing molecular mechanisms.