Protective Activity of <i>Markhamia tomentosa</i>(Benth.) K. Schum. (Bignoniaceae) Methanol Leaves Extract against D-Galactosamine/Lipopolysaccharide-Induced Acute Liver Injury in Mice

作     者：Roméo Joel Guemmogne Temdie Agathe Lambou Fotio Flaure Donfack Metchi Edwige Chiogo Vouffo Ymele Gilbert Thierry Nkoulou Tabi Théophile Dimo Roméo Joel Guemmogne Temdie;Agathe Lambou Fotio;Flaure Donfack Metchi;Edwige Chiogo Vouffo Ymele;Gilbert Thierry Nkoulou Tabi;Théophile Dimo

作者机构：Department of Biological Sciences Faculty of Science University of Ngaoundere Ngaoundere Cameroon Department of Zoology and Animal Physiology Faculty of Science University of Buea Buea Cameroon Department of Animal Biology and Physiology Faculty of Science University of Yaounde I Yaounde Cameroon 

出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)

年 卷 期：2020年第8卷第10期

页      面：74-89页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Markhamia tomentosa D-Galactosamine/Lipopolysaccharide Oxidative Stress Liver Injury 

摘      要：Markhamia tomentosa (Benth.) K. Schum. (Mt) is a Cameroonian medicinal plant, traditionally used to treat painful and inflammatory illness. This study aimed to examine the effects of methanol leaves extract (MLE) of Mt in D-galactosamine (D-GaIN)/lipopolysaccharide (LPS)-induced liver injury. The MLE (100 and 200 mg/kg), Ascorbic acid (10 mg/kg) and distilled water were administered 12 h and 1 h before intraperitoneal injection of D-GaIN (10 mg/mouse)/LPS (0.1 μg/g). Animals were sacrificed 6 h after D-GalN/LPS challenge. Liver injury was assessed biochemically by determination of aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), alkaline phosphatase (ALP), superoxide dismutase (SOD) and catalase activities. Malondialdehyde (MDA), reduced glutathione (GSH), nitrites, total protein and bilirubin levels were explored. Histopathological examination of liver tissue was also performed. Liver enzymes (ALAT, ASAT, ALP) activity, nitrites, MDA and bilirubin levels were increased, while protein level, SOD and catalase activities were significantly reduced by D-GalN/LPS administration. MLE (100 or 200 mg/kg) protected mice against D-GalN/LPS-induced death. In addition, the plant extract significantly reduced ALAT and ALP activity, exhibiting 23.00% and 62.20% protection, respectively. SOD activity and total protein were significantly (p -GaIN/LPS-induced liver injury. The obtained results