Exploring novel cell cryoprotectants based on neutral amino acids

作     者：Xiaojie Sui Pengguang Chen Chiyu Wen Jing Yang Qingsi Li Lei Zhang Xiaojie Sui;Pengguang Chen;Chiyu Wen;Jing Yang;Qingsi Li;Lei Zhang

作者机构：Department of Biochemical EngineeringSchool of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China Qingdao Institute for Marine Technology of Tianjin UniversityQingdao 266235China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2020年第28卷第10期

页      面：2640-2649页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：the financial support from the National Natural Science Foundation of China(Nos.21621004,21961132005,21908160 and 21422605) the Qingdao National Laboratory for Marine Science and Technology(QNLM2016ORP0407) the Tianjin Natural Science Foundation(18JCYBJC29500) the China Postdoctoral Science Foundation(2019M651041) 

主　　题：Biomedical engineering Cell engineering Tissue engineering Cryopreservation Cryoprotectant Neutral amino acid 

摘      要：Cryoprotectants play a key role in cell cryopreservation because they can reduce cryoinjuries to cells associated with ice *** meet the clinical requirements of cryopreserved cells,cryoprotectants should be biocompatible,highly efficient and easily removable from cryopreserved ***,integration of these properties into one cryoprotectant still remains ***,three biocompatible neutral amino acids,includingβ-alanine,γ-aminobutyric acid andε-aminocaproic acid,are first reported to have the potential as such ideal *** results demonstrate that they can inhibit ice formation and reduce osmotic stress to provide extracellular and intracellular protection,thereby ensuring high cryopreservation efficiency for both anuclear and nucleated *** importantly,due to the remarkable osmotic regulation ability,the neutral amino acids can be rapidly removed from cryopreserved cells via a one-step method without causing observable damage to cells,superior to the current state-of-the-art cryoprotectants—dimethyl sulfoxide and *** work provides a new perspective to develop novel cryoprotectants,which may have dramatic impacts on solvent-free cryopreservation technology to support the cell-based applications,such as cell therapy and tissue engineering,etc.